Basic Research Research Articles

Full-scale experimental, numerical, and theoretical research on the spatio-temporal evolution of the flow structure in longitudinal and semi-transversal ventilated tunnels

This paper presents full-scale experimental, numerical, and theoretical research on the spatial and temporal structure of airflow for different modes of ventilation system operation in a road tunnel. The tunnel under investigation is equipped with a semi-transverse ventilation system. There are 19 pairs of axial jet fans in each tunnel tube and 164 air supply vents on both sides of a tube located just above the road surface. A set of seven stand poles with mounted vane anemometers were arranged along the tunnel tube axis. Different configurations of axial jet fans and air supply vents were employed, and changes in airflow velocity were recorded. Since the main objective of the study is to achieve a deeper understanding of the examined phenomena, a numerical model of the flows in the tunnel was built, and the acquired data was used to validate it. Some theoretical considerations are provided and also validated. All of this together reveals a very uneven distribution of longitudinal air velocity along the tunnel axis. Then, time constants when switching the fans on and off are determined. It is shown that the time needed to achieve a stable flow after the fans are turned on is in the order of five minutes, and the decay time after the fans are turned off is much longer; it takes about 15 min to calm down the flow. Next, it is determined that the transverse air supply significantly changes the airflow structure: the average longitudinal air velocity at the inlet portal was up to 25% lower than without it, while values at the outlet portal were similar. Finally, it is indicated that axial fans that operate in the suction mode are similarly efficient to those in the pumping mode.

VHL mutation drives human clear cell renal cell carcinoma progression through PI3K/AKT-dependent cholesteryl ester accumulation

Cholesteryl ester (CE) accumulation in intracellular lipid droplets (LDs) is an essential signature of clear cell renal cell carcinoma (ccRCC), but its molecular mechanism and pathological significance remain elusive. Enabled by the label-free Raman spectromicroscopy, which integrated stimulated Raman scattering microscopy with confocal Raman spectroscopy on the same platform, we quantitatively analyzed LD distribution and composition at the single cell level in intact ccRCC cell and tissue specimens in situ without any processing or exogenous labeling. Since we found that commonly used ccRCC cell lines actually did not show the CE-rich signature, primary cancer cells were isolated from human tissues to retain the lipid signature of ccRCC with CE level as high as the original tissue, which offers a preferable cell model for the study of cholesterol metabolism in ccRCC. Moreover, we established a patient-derived xenograft (PDX) mouse model that retained the CE-rich phenotype of human ccRCC. Surprisingly, our results revealed that CE accumulation was induced by tumor suppressor VHL mutation, the most common mutation of ccRCC. Moreover, VHL mutation was found to promote CE accumulation by upregulating HIFα and subsequent PI3K/AKT/mTOR/SREBPs pathway. Inspiringly, inhibition of cholesterol esterification remarkably suppressed ccRCC aggressiveness invitro and invivo with negligible toxicity, through the reduced membrane cholesterol-mediated downregulations of integrin and MAPK signaling pathways. Collectively, our study improves current understanding of the role of CE accumulation in ccRCC and opens up new opportunities for treatment. This work was supported by National Natural Science Foundation of China (No. U23B2046 and No. 62027824), National Key R&D Program of China (No. 2023YFC2415500), Fundamental Research Funds for the Central Universities (No. YWF-22-L-547), PKU-Baidu Fund (No. 2020BD033), Peking University First Hospital Scientific and Technological Achievement Transformation Incubation Guidance Fund (No. 2022CX02), and Beijing Municipal Health Commission (No. 2020-2Z-40713).

Substitution Of Potato Flour In Mangkok Cake Making

This research aims to analyze the effect of potato starch substitution on the quality of cupcakes by adding 20%, 40% and 60% potato starch. This type of research is pure experimental research with a completely randomized design (CRD) method consisting of 4 treatments and 3 repetitions. The object of this research is cupcakes with potato starch substitution of 20%, 40%, 60% for the quality of the cupcakes. The data collection technique for this research was carried out using organoleptic tests given to 5 expert panelists, namely lecturers in Family Welfare Science, Padang State University. The independent variable in this research is potato flour. The dependent variable of this research is the quality of the cupcakes produced including shape, color, aroma, texture and taste. The data analysis technique used is Analysis of Variance (ANOVA) to analyze organoleptic test data. The data that has been obtained is then tabulated in table form and analyzed according to each data test. Then data analysis is carried out, if the data obtained by Fcount is greater than Ftable then proceed with the Duncan test. The results of this research show that there is a real influence on the quality of texture and taste of cupcakes, while there is no significant difference in the quality of shape, aroma and color. It can be concluded that the best quality test results for potato flour cupcakes were in treatment (X3) with 40% potato starch substitution. 

Community Response to Internet Addiction among Minors: An Overview, Findings, Challenges

Purpose: The article aims to explore the nature and characteristics of minors' Internet addiction behavior, and analyze the comprehensive factors as well as the diverse effects based on the deviant behavior theory. The deviant behavior theory, integrating misconduct with sociocultural concepts, social values, and norms, can provide new insight into the existing theoretical research on this issue.

Smart materials for light control of droplets.

Droplet manipulation plays a critical role in both fundamental research and practical applications, especially when combined with smart materials and external fields to achieve multifunctional droplet manipulation. Light control of droplets has emerged as a significant and widely used strategy, driven primarily by photochemistry, photomechanics, light-induced Marangoni effects, and light-induced electric effects. This approach allowing for droplet manipulation with high spatial and temporal resolution, all while maintaining a remote and non-contact mode of operation. This review aims to provide a comprehensive overview of the mechanisms underlying light control of droplets, the design of smart materials for this purpose, and the diverse range of applications enabled by this technique. These applications include merging, splitting, releasing, forwarding, backward movement, and rotation of droplets, as well as chemical reactions, droplet robots, and microfluidics. By presenting this information, we aim to establish a unified framework that guides the sustainable development of light control of droplets. Additionally, this review addresses the challenges associated with light control of droplets and suggests potential directions for future development.

Holistic entrepreneurial inventory: a framework for the integration of spirituality in entrepreneurial performanceInventario emprendedor holístico: un marco para la integración de la espiritualidad en el desempeño emprendedorInventário emprendedor holístico: um framework para a integração da espiritualidade no desempenho emprendedor

Purpose This study aims to explore the constructs of spirituality (SP) and entrepreneurial performance (EP) and their measurements to identify their connections. Attending to the needs of entrepreneurs to face the current global crisis, this research establishes a framework that integrates SP to enhance EP. Design/methodology/approach This study focuses on a comprehensive literature review and a comparison between constructs to come across with the proposal of a conceptual framework. Findings For SP, spiritual well-being was found as a good measure which, to the best of the authors’ knowledge, has never been used in entrepreneurship. EP has been typically measured by economic indicators, but recently, noneconomic benefits and effects on stakeholders have also been considered, so an integrated approach is proposed. The main contribution is a framework called holistic entrepreneurial inventory (HEI), which integrates the main elements of the constructs in a matrix showing their interconnection. It is accompanied by a scorecard for entrepreneurs to identify strengths and weaknesses in SP and EP. Research limitations/implications This is a theoretical research and proposal; further empirical research could help confirm the present conceptual findings. Practical implications To help entrepreneurs identify the areas where they would like to improve, understanding the connection between SP and EP, which are dynamic states in constant change. Originality/value EP has been mainly focused on exogenous causes. The HEI scorecard may give entrepreneurs a different perspective from the innermost part of their being.

Approach to Spirocyclohexadienes via Visible Light‐Mediated ipso‐Cyclization of Amino Acid Derivatives with N‐(2‐Phenyl)benzoyl Groups

Spirocycles are important structural motifs, found in natural and pharmaceutical compounds, which have attracted considerable interest in modern drug discovery research. Herein, we describe the development of a visible light‐mediated ipso‐cyclization of amino acid derivatives with N‐(2‐phenyl)benzoyl groups, allowing easy access to a variety of spirocyclohexadienes. The addition of water was found to be beneficial in promoting the reaction progress. Investigation of the substrate scope revealed that incorporating an electron‐donating moiety at the 3'‐position of the biphenyl group resulted in a more favorable outcome for spirocycle formation.

Thickness‐Dependent Crystal Structure, Synthesis, and Magnetism of Thin Film Chromium Chalcogenides: A Review

AbstractIn the search for two‐dimensional (2D) magnets operating under ambient conditions, quasi‐2D non‐van der Waals (vdW) materials have attracted considerable research interest over the last five years. In addition to their high Curie temperature (TC), their superior air stability to that of vdW 2D magnets has made them potential candidates for future room‐temperature spintronics applications. Furthermore, recent progress in the thickness‐dependent crystal lattice and magnetic properties of non‐vdW Cr2X3 (X = S, Se, or Te) has brought them to areas that are once set aside for 2D vdW magnets in fundamental research and applications. Despite covalent bonding between magnetic layers, various bottom‐up synthesis methods produced thickness‐controlled flakes of Cr2X3. Moreover, layer‐dependent structural and magnetic properties are among the novel research directions in these materials. This review systematically summarizes recent studies on Cr2X3 crystal structure, their controllable synthesis, and their respective magnetic properties. A summary of some significant results on thickness‐controlled synthesis in Cr2X3 and thickness‐dependent magnetism in Cr2Te3 is presented. Additionally, experimental and theoretical reports are presented to explain interlayer magnetic interaction. The work reveals the interaction between synthesis, structure, and magnetism. Finally, future research directions of new Cr2X3‐based materials, rich magnetism in Cr2X3, and their potential application are discussed.

Fully automated operational modal identification based on scale-space peak picking algorithm and power spectral density estimation

Modal analysis is a fundamental and essential research direction in the field of structural engineering. The ultimate goal is to determine the modal parameters of the structures. However, the existing modal analysis algorithms often require a large number of parameter adjustments and manual intervention during operation, which cannot be fully automated. In order to realize the automatic identification of modal parameters, the automatic operational modal identification method (AOMI) is proposed based on the interpolated power spectral density estimation (IPSE). To achieve more precise spectrum analysis in the low-frequency band, IPSE is employed to perform Fourier transform on the original frequency domain segment with optimized frequency resolution. This enhances the sharpness of the obtained spectrum in the low-frequency range, making peak frequencies more discernible. Subsequently, the scale-space peak picking algorithm is used to automatically obtain the peak of the power spectral density (PSD), thus enabling the automatic identification of the natural frequency. Finally, the frequency domain decomposition method (FDD) is used to identify modal parameters based on the natural frequencies. The effectiveness of AOMI is verified through the modal identification of the old steel truss bridge and the three layer framework. Under the environmental excitation, the frequencies identified by the IPSE method is close to that of FDD, Bayesian fast fourier transform (FFT) and covariance driven stochastic subspace identification (SSI-COV). Furthermore, the PSD obtained through IPSE has sharper peak than that of FDD and the Welch’s method. The damping ratio identification accuracy and modal assurance criterion (MAC) are satisfactory in AOMI, which can improve the automatic performance.

Bridging the gap between academic research and industrial development in advanced all-solid-state lithium-sulfur batteries.

The energy storage and vehicle industries are heavily investing in advancing all-solid-state batteries to overcome critical limitations in existing liquid electrolyte-based lithium-ion batteries, specifically focusing on mitigating fire hazards and improving energy density. All-solid-state lithium-sulfur batteries (ASSLSBs), featuring earth-abundant sulfur cathodes, high-capacity metallic lithium anodes, and non-flammable solid electrolytes, hold significant promise. Despite these appealing advantages, persistent challenges like sluggish sulfur redox kinetics, lithium metal failure, solid electrolyte degradation, and manufacturing complexities hinder their practical use. To facilitate the transition of these technologies to an industrial scale, bridging the gap between fundamental scientific research and applied R&D activities is crucial. Our review will address the inherent challenges in cell chemistries within ASSLSBs, explore advanced characterization techniques, and delve into innovative cell structure designs. Furthermore, we will provide an overview of the recent trends in R&D and investment activities from both academia and industry. Building on the fundamental understandings and significant progress that has been made thus far, our objective is to motivate the battery community to advance ASSLSBs in a practical direction and propel the industrialized process.

The Relevance Of <i>Hifdzun Nafs </i>With The Law of <i>Qishash:</i> An Analysis Of Ibnu 'Ashur's Interpretation In <i>Tafsir al-Tahrir Wa al-Tanwir </i>

This article aims to determine the relevance of hifdzun nafs to the law of qishash from Ibn ‘Asyur’s perspective in the tafsir al-Tahrir wa al-Tanwir. Ibn ‘Asyur himself was famous as a mufasir who focused on Maqashid al-Syari’ah so that he also expressed the results of his thoughts about Maqashid in the form of interpretation. By using qualitative methods, pure literature research, this article concludes that according to Ibn ‘Asyur, qishash functions to protect human life, because with qishash people are afraid of committing murder. If a person knows that he will survive death, he will dare to commit murder in the hope that the punishment will be light. Therefore, the qishash law that was implemented has strong relevance to hifduzn nafs, namely avoiding bloodshed as in the case of the murders that occurred during the Jahiliyyah era, which resulted in the loss of many lives. The qishash law is one way to protect the soul (hifdzun nafs), by punishing people who commit murder and other criminal acts, with appropriate punishments for the benefit and survival of humans.

Erratum to: Molecular dynamics simulation reveals DNA-specific recognition mechanism via c-Myb in pseudo-palindromic consensus of mim-1 promoter.

The original version of this article (Weng et al., 2023) unfortunately contained a mistake. In Acknowledgments, the number (No. 226-2022-00213) of the Fundamental Research Funds for the Central Universities is wrong. The correct number should be No. 2022FZZX01-33.

Exploring stem cell frontiers: definitions, challenges, and perspectives for regenerative medicine.

Each year, the European Summer School on Stem Cell Biology and Regenerative Medicine (SCSS) attracts early-career researchers and actively practicing clinicians who specialise in stem cell and regenerative biology. The 16th edition of this influential course took place from 12th to 19th September 2023 on the charming Greek island of Spetses. Focusing on important concepts and recent advances in stem cells, the distinguished faculty included experts spanning the spectrum from fundamental research to clinical trials to market-approved therapies. Alongside an academically intensive programme that bridges the various contexts of stem cell research, delegates were encouraged to critically address relevant questions in stem cell biology and medicine, including broader societal implications. Here, we present a comprehensive overview and key highlights from the SCSS 2023.

Endohedral Fullerenes: From Exotic Chemical Bonding to Molecular Circuits

Since their discovery, fullerenes represent a fascina­ting type of molecule with many unusual properties. One of these is the existence of a cavity into which other atoms and molecules can be enclosed to form the so-called endohedral fullerenes. In terms of fundamental research, endohedral fullerenes provide a platform for studying exotic chemical bonds between atoms enclosed inside the fullerene cage. At the same time, endohedral fullerenes also find practical applications, for example in solar cells, material science, electronics and molecular electronics. In this article, we review the research on exotic bonds in fullerenes and the use of fullerenes as molecular components.

Metabolically Tailored Selection of Ornamental Rose Cultivars through Polyamine Profiling, Osmolyte Quantification and Evaluation of Antioxidant Activities

Roses (genus Rosa), renowned for their economic significance and aesthetic appeal, face multifaceted challenges in cultivation due to biotic and abiotic stressors. To address these challenges, this study explores the role of osmolytes, particularly polyamines, proline and glycine betaine, as well as antioxidant capacities and condensed tannins, in enhancing stress tolerance in roses. Despite the genetic diversity inherent in roses, the metabolic aspect of stress tolerance has been underexplored in breeding programs. This paper investigates the intraspecific variability among 22 rose cultivars, focusing on osmolyte content (proline and glycine betaine), individual polyamines (putrescine, spermine and spermidine), as well as antioxidant activities, measuring radical scavenging capacity against 2,2′-azinobis(3-ethylbenzothiozoline-6-sulfonic acid (ABTS•+) and NO• radicals. Employing a targeted metabolomic approach, we quantified the levels of individual polyamines in both the petals and leaves of rose cultivars. This was achieved through high-performance liquid chromatography coupled with fluorescent detection following a derivatization pretreatment process. Within the evaluated cultivars, “Unique Aroma”, “Andre Rieu”, “Aroma 3”, “Frayla Marija” and “Trendy Fashion” stood out for their significantly elevated levels of total foliar polyamines. The predominant polyamine detected at both petal and leaf levels was putrescine, with concentrations ranging from 335.81 (“Zora Frayla”) to 2063.81 nmol g−1 DW (“Unique Aroma”) at the leaf level. Following putrescine, foliar spermidine levels varied from 245.08 (“Olivera Frayla”) to 1527.16 nmol g−1 DW (“Andre Rieu”). Regarding antioxidant capacity, the leaf extracts of rose cultivars “Zora Frayla” and “Natalija Frayla” were prominent by showing 68.08 and 59.24 mmol Trolox equivalents (TE) g−1 DW, respectively. The results highlight the intricate biochemical variability across rose cultivars and show that osmolytes, such as glycine betaine, proline and polyamines, and other biochemical markers can be used as reliable criteria for the selection of rose cultivars that are more resilient to biotic stress factors, especially powdery and downy mildew. Bridging fundamental research with practical applications, this study aims to contribute to the development of stress-tolerant rose cultivars adaptable to dynamic environmental conditions.

A simultaneous measurement method for multi-directional galloping of iced bundled conductors and its application in spatial galloping behavior

Abstract The theoretical results indicate that iced bundled conductors experience spatial gallop-ing due to wind-induced vibration, involving in-plane, out-of-plane, and torsional movements. To better understand the dynamic response of this behavior from an exper-imental perspective, an innovative experimental method has been proposed. The method can simultaneously measure the in-plane, out-of-plane, and torsional vibration signals of iced bundled conductors’ galloping. A testing system was established, and the method is applied in the galloping experiment of continuous iced bundled conductors, validating some theoretical results. This paper describes the construction of a wide-aperture, low-speed wind tunnel suitable for testing the galloping of continuous iced bundled conduc-tors. A “cut-bury-glue” method was proposed to create a model of continuous iced bun-dled conductors effectively, along with a method for connecting subconductors to im-prove experimental precision. The tests utilized laser displacement sensors and wireless posture sensors, considering the installation and data collection characteristics of the sensors. Through signal conversion, error correction, and other technical methods, sim-ultaneous measurement of in-plane, out-of-plane, and torsional vibration signals was achieved. The spatial galloping behavior, changing with wind speed, exhibits limited-amplitude and synchronous characteristics. The participation of different modes shows elliptical orbital motion in single-mode galloping and “8” shaped orbital motion in cou-pled-mode galloping. These results are consistent with previous theoretical research, offering a new approach to studying iced bundled conductors’ galloping.

Multi-Scenario Simulation and Eco-Environmental Effects Analysis of Land Use/Cover Change in China by an Integrated Cellular Automata and Markov Model

Land use transitions play a critical role in ecological environmental restoration, but they are also plagued by ecological environmental problems caused by excessive land resource development. In this study, we propose a methodological framework for unveiling the nexus profile of land use/cover change (LUCC) and eco-environmental effects. This study explored the spatiotemporal evolution patterns of LUCC over a long time series based on high-precision land use data from 1990 to 2020. Then, the ecological values (EVs) of various cities were calculated to obtain the ecological contribution rate of different land use types in the process of change. Finally, the future development trends of land use and ecological environmental quality were predicted under multiple scenarios using the cellular automata–Markov model, and scientific policy recommendations were proposed. The results showed that the expansion trajectory of the construction land in the urban agglomeration mainly expanded inwards along the mouth of the Pearl River, and the conversion of cultivated land to construction land was the most significant type of land use change. The overall ecological environmental quality of the study area showed a downwards trend, with Shenzhen exhibiting the largest decrease in EVs. Cultivated land contributed significantly to improving regional ecological environmental quality, while the land use transition types with relatively large contributions to environmental quality deterioration were conversions to construction land. Under the scenario of coordinated protection, the degree of cultivated land area reduction was significantly reduced, and the area of forestland showed a positive growth trend, with the expansion trend of construction land being reversed. These research findings can enrich the theoretical research on the sustainable development of urban agglomerations and provide reliable data support for policy-making.

The concept of performative national identity: the theoretical methodological framework

   Modern social and political changes emphasize the particular relevance of the problem of forming national identity in the context of often contradictory sociocultural processes. Leveling out negative social phenomena that exploit the category of national identity and developing its potential for the formation of civic values and harmonization of interethnic relations in Russian society necessitates a deep understanding of this phenomenon, which is considered primarily as a result of its construction by social institutions.   However, this process also takes the form of assimilation of elements of nation-building, passed through the filters of personal attitudes, representation of individual national identity in various social situations, which constitutes the problem of this research.   Based on the study of theoretical data, the author proposed a category of performative national identity based on the application of the approaches of social constructivism and symbolic interactionism. The methods of theoretical research were theoretical analysis and synthesis, induction and modeling. As a result, the category of performative national identity has been developed, which includes technologies, features, resources for construction and conscious and unconscious representation of national identity in everyday practices. The results of the research develop the theory and methodology of constructing national identity, expand the understanding of the process of formation of national identity as a result of the assimilation of collective memory through the prism of personal attitudes, and also allow us to study the features of the representation of national identity in various situations from the point of view of the dramaturgical approach.

Research on the coordinated development of provincial urbanization and carbon emission efficiency of construction industry in China

BackgroundEnergy conservation and emission reduction policies restrict the economic and social development of all countries in the world, and the impact on China, which has low urbanization, is more serious. In the process of promoting urbanization, the pressure of carbon emission reduction in the construction industry has increased, and the high emissions of the construction industry have made the low-carbon development of cities face severe challenges. China is at a critical stage of urbanization development, and there is become a general consensus on how to improve the carbon emission efficiency of the construction industry. The interaction between urbanization and the carbon emission efficiency of the construction industry is a long-term and complex process. As one of the industries contributing to China’s urbanization process and carbon emissions, it is of great practical significance to explore the coordination relationship between urbanization and the carbon emission efficiency of the construction industry (CEECI) to realize the goal of “double carbon”, promoting urbanization construction and solving the problem of “green development”. Taking 30 provinces in China as the research target area, the double weighted summation method and the undesirable output superefficiency window-EBM-DEA model are used to measure the provincial urbanization level and CEECI, respectively. Then, the coupling coordination degree model of the relative development index is introduced, and the spatial autocorrelation model and the spatial and temporal differentiation characteristics of the coordination level of urbanization and the CEECI are analysed.ResultsFrom 2010 to 2021, China’s urbanization level increased steadily, but the growth rate gradually decreased. There were significant differences in urbanization levels among provinces. The eastern provinces have a higher level of urbanization but lack an impetus in the later period, while the western provinces have a lower level of urbanization but a faster growth rate. The low-carbon development trend of China’s construction industry is good, and the overall development of the CEECI shows an “N” type, and the inflection points appear in 2013 and 2018. The interprovincial urbanization level is significantly different from that of the CEECI, and the development form of the central and western provinces is better than that of the eastern provinces. The coordination level of urbanization and the CEECI in China is transitioning from the running-in stage to the coordination stage, and the coupling coordination degree between systems is on the rise, while the relative development degree is on the decline. The spatial distribution pattern is in a dynamic state of change, and the overall distribution pattern is “high in the east and low in the central and western regions”. The differences among provinces were significantly decreased, with 63.33% of provinces at the high running-in level. The provinces that entered the coordination stage were mainly located in the eastern region, and only Beijing was in the coordination stage by the end of the study. In addition, 90% of the provinces exhibited lagging efficiency, and only Jiangxi, Guangxi and Chongqing, which had low coordination levels maintained synchronous development of the two systems. The coordination level between provincial urbanization and the CEECI showed a positive spatial distribution, the global Moran index showed a “V” shape trend, and the spatial dependence of the coordination level between the two systems gradually weakened. In the local spatial distribution, there are two types of convergence: high and low.ConclusionsThe coordination degree of urbanization and the CEECI analysed in this study is an extension of the research on the relationship between the two. By integrating the two into a unified framework, the method of combining quantitative and qualitative analysis is used to further explore the coordination relationship between the two, which not only enhances the scientificity and accuracy of the research but also extends the breadth and depth of relevant theoretical research. At the same time, according to the coordination type between urbanization and the CEECI, China should propose corresponding targeted coordination and optimization paths from the perspective of urbanization and low-carbon development of the construction industry to achieve high-quality development of China’s economy and society.

Discovery of Strong 3-Nitro-2-Phenyl-2H-Chromene Analogues as Antitrypanosomal Agents and Inhibitors of Trypanosoma cruzi Glucokinase

Chagas disease is one of the world’s neglected tropical diseases, caused by the human pathogenic protozoan parasite Trypanosoma cruzi. There is currently a lack of effective and tolerable clinically available therapeutics to treat this life-threatening illness and the discovery of modern alternative options is an urgent matter. T. cruzi glucokinase (TcGlcK) is a potential drug target because its product, d-glucose-6-phosphate, serves as a key metabolite in the pentose phosphate pathway, glycolysis, and gluconeogenesis. In 2019, we identified a novel cluster of TcGlcK inhibitors that also exhibited anti-T. cruzi efficacy called the 3-nitro-2-phenyl-2H-chromene analogues. This was achieved by performing a target-based high-throughput screening (HTS) campaign of 13,040 compounds. The selection criteria were based on first determining which compounds strongly inhibited TcGlcK in a primary screen, followed by establishing on-target confirmed hits from a confirmatory assay. Compounds that exhibited notable in vitro trypanocidal activity over the T. cruzi infective form (trypomastigotes and intracellular amastigotes) co-cultured in NIH-3T3 mammalian host cells, as well as having revealed low NIH-3T3 cytotoxicity, were further considered. Compounds GLK2-003 and GLK2-004 were determined to inhibit TcGlcK quite well with IC50 values of 6.1 µM and 4.8 µM, respectively. Illuminated by these findings, we herein screened a small compound library consisting of thirteen commercially available 3-nitro-2-phenyl-2H-chromene analogues, two of which were GLK2-003 and GLK2-004 (compounds 1 and 9, respectively). Twelve of these compounds had a one-point change from the chemical structure of GLK2-003. The analogues were run through a similar primary screening and confirmatory assay protocol to our previous HTS campaign. Subsequently, three in vitro biological assays were performed where compounds were screened against (a) T. cruzi (Tulahuen strain) infective form co-cultured within NIH-3T3 cells, (b) T. brucei brucei (427 strain) bloodstream form, and (c) NIH-3T3 host cells alone. We report on the TcGlcK inhibitor constant determinations, mode of enzyme inhibition, in vitro antitrypanosomal IC50 determinations, and an assessment of structure–activity relationships. Our results reveal that the 3-nitro-2-phenyl-2H-chromene scaffold holds promise and can be further optimized for both Chagas disease and human African trypanosomiasis early-stage drug discovery research.